| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
10/07/2019 |
|
Actualizado : |
26/08/2019 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
HERNÁNDEZ-RODRÍGUEZ, L.; BENÍTEZ-GALEANO, M.J.; BERTALMIO, A.; RUBIO, L.; RIVAS, F.; ARRUABARRENA, A.; ROLON, R.; COLINA, R.; MAESO, D. |
|
Afiliación : |
LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República, Salto, Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO ROQUE ROLON RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República, Salto, Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Diversity of Uruguayan citrus tristeza virus populations segregated after single aphid transmission. |
|
Fecha de publicación : |
2019 |
|
Fuente / Imprenta : |
Tropical Plant Pathology, 2019, volume 44, Issue 4, pages 352-362 |
|
ISSN : |
1983-2052 |
|
DOI : |
10.1007/s40858-019-00288-x |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 16 November 2018 / Accepted 10 April 2019 / First Online 06 May 2019 // Published 15 August 2019.
Acknowledgments: This research was funded by the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay (project CT-06), through the National Program of Citrus Research and the National Citrus Sanitation Program. |
|
Contenido : |
ABSTRACT.
Prevalence of citrus tristeza virus (CTV) isolates causing severe stem pitting symptoms with a high diverse genetic composition represents a threat for citrus crops in Uruguay. This work aimed to characterize viral components of CTV populations segregated by single aphid (Toxoptera citricida) transmission (SAT). Thirty-nine SAT-derived sub-isolates were obtained from six CTV field isolates. Only eight were MCA13 non-reactive, and six of them, together with other three MCA13 reactive sub-isolates, were considered mild by the intensity of their reaction in ?Mexican? lime, ?Madam Vinous? sweet orange, ?Duncan? grapefruit and sour orange indicator plants. Fourteen sub-isolates, including two MCA13 non-reactive, were considered moderate, and the remaining sub-isolates were all MCA13 reactive and with severe reactions in the indicator plants. Sequence comparisons of the p20, p23 and p25 genes showed a high inter (among all isolates and their derived sub-isolates)- and intra (among each isolate and its derived sub-isolates)-specific evolutionary diversity, with the presence of the CTV strains VT, T3, RB, and NC in mixtures of their complex populations. Together, our data revealed the heterogeneity of the Uruguayan CTV populations in these CTV field isolates. The results provide additional biological and molecular information on the complex CTV populations in Uruguay and must be considered for improving the CTV management program.
© Sociedade Brasileira de Fitopatologia 2019 MenosABSTRACT.
Prevalence of citrus tristeza virus (CTV) isolates causing severe stem pitting symptoms with a high diverse genetic composition represents a threat for citrus crops in Uruguay. This work aimed to characterize viral components of CTV populations segregated by single aphid (Toxoptera citricida) transmission (SAT). Thirty-nine SAT-derived sub-isolates were obtained from six CTV field isolates. Only eight were MCA13 non-reactive, and six of them, together with other three MCA13 reactive sub-isolates, were considered mild by the intensity of their reaction in ?Mexican? lime, ?Madam Vinous? sweet orange, ?Duncan? grapefruit and sour orange indicator plants. Fourteen sub-isolates, including two MCA13 non-reactive, were considered moderate, and the remaining sub-isolates were all MCA13 reactive and with severe reactions in the indicator plants. Sequence comparisons of the p20, p23 and p25 genes showed a high inter (among all isolates and their derived sub-isolates)- and intra (among each isolate and its derived sub-isolates)-specific evolutionary diversity, with the presence of the CTV strains VT, T3, RB, and NC in mixtures of their complex populations. Together, our data revealed the heterogeneity of the Uruguayan CTV populations in these CTV field isolates. The results provide additional biological and molecular information on the complex CTV populations in Uruguay and must be considered for improving the CTV management program.
© Sociedade Brasileira de Fitopatologia 20... Presentar Todo |
|
Palabras claves : |
Biological indexing; Closterovirus; CTV; SAT; Toxoptera citricida. |
|
Asunto categoría : |
F01 Cultivo |
|
Marc : |
LEADER 02739naa a2200313 a 4500
001 1059942
005 2019-08-26
008 2019 bl uuuu u00u1 u #d
022 $a1983-2052
024 7 $a10.1007/s40858-019-00288-x$2DOI
100 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
245 $aDiversity of Uruguayan citrus tristeza virus populations segregated after single aphid transmission.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 16 November 2018 / Accepted 10 April 2019 / First Online 06 May 2019 // Published 15 August 2019. Acknowledgments: This research was funded by the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay (project CT-06), through the National Program of Citrus Research and the National Citrus Sanitation Program.
520 $aABSTRACT. Prevalence of citrus tristeza virus (CTV) isolates causing severe stem pitting symptoms with a high diverse genetic composition represents a threat for citrus crops in Uruguay. This work aimed to characterize viral components of CTV populations segregated by single aphid (Toxoptera citricida) transmission (SAT). Thirty-nine SAT-derived sub-isolates were obtained from six CTV field isolates. Only eight were MCA13 non-reactive, and six of them, together with other three MCA13 reactive sub-isolates, were considered mild by the intensity of their reaction in ?Mexican? lime, ?Madam Vinous? sweet orange, ?Duncan? grapefruit and sour orange indicator plants. Fourteen sub-isolates, including two MCA13 non-reactive, were considered moderate, and the remaining sub-isolates were all MCA13 reactive and with severe reactions in the indicator plants. Sequence comparisons of the p20, p23 and p25 genes showed a high inter (among all isolates and their derived sub-isolates)- and intra (among each isolate and its derived sub-isolates)-specific evolutionary diversity, with the presence of the CTV strains VT, T3, RB, and NC in mixtures of their complex populations. Together, our data revealed the heterogeneity of the Uruguayan CTV populations in these CTV field isolates. The results provide additional biological and molecular information on the complex CTV populations in Uruguay and must be considered for improving the CTV management program. © Sociedade Brasileira de Fitopatologia 2019
653 $aBiological indexing
653 $aClosterovirus
653 $aCTV
653 $aSAT
653 $aToxoptera citricida
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aBERTALMIO, A.
700 1 $aRUBIO, L.
700 1 $aRIVAS, F.
700 1 $aARRUABARRENA, A.
700 1 $aROLON, R.
700 1 $aCOLINA, R.
700 1 $aMAESO, D.
773 $tTropical Plant Pathology, 2019, volume 44, Issue 4, pages 352-362
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
|
Fecha actual : |
16/11/2015 |
|
Actualizado : |
15/10/2019 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
A - 1 |
|
Autor : |
MEDEROS, A.; KELTON, D.; PEREGRINE, A.S.; VANLEEUWEN, J.; FERNÁNDEZ, S.; LEBOEUF, A.; MENZIES, P.; MARTIN, R. |
|
Afiliación : |
AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Evaluation of the utility of subjective clinical parameters for estimating fecal egg counts and packed cell volume in Canadian sheep flocks. |
|
Fecha de publicación : |
2014 |
|
Fuente / Imprenta : |
Veterinary Parasitology, 2014, v.205, p. 568?574. |
|
ISSN : |
0304-4017 |
|
DOI : |
10.1016/j.vetpar.2014.08.030 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 12 February 2014 / Received in revised form 8 August 2014 / Accepted 30 August 2014. |
|
Contenido : |
ABSTRACT.
A study was conducted in sheep on Canadian farms to describe the relationship between packed cell volume (PCV) or fecal egg counts (FEC) and subjective clinical parameters that
may indicate the severity of parasitic gastroenteritis. Twenty-one farms in Ontario (ON) and 8 farms in Quebec (QC) were purposively selected and visited during April?May (spring)
and August (summer) 2007. At each farm visit, blood and fecal samples were collected from 10 ewes and 10 female lambs; body condition score (BCS), dag score (DS), fecal consistency score (FCS) and FAMACHA score were recorded for all sampled sheep. Packed cell volume was determined for all blood samples, and FEC were performed for all fecal samples. Summary statistics and simple correlations were performed for the parameters recorded. Two mixed models with random effects at the farm level were developed; one using PCV as the response variable and another using the natural log of eggs per gram of feces (lnEPG). Finally, the residuals from both models were correlated to the covariates in the models. The mean PCV values during the spring were 29.7% and 36.7% for lambs, and 28.8% and 31.1% for ewes, in ON and QC, respectively. During the summer, the mean PCV was 32.0% and 32.8% for lambs, and 30.1% and 29.9% for ewes, in ON and QC, respectively. The arithmetic mean FEC per gram of feces (EPG) during the spring was 3 and 2 for lambs, and 1266 and 789 for ewes, in ON and QC, respectively, whereas during summer the arithmetic mean EPG was 907 and 237 for lambs, and 458 and 246 for ewes, in ON and QC, respectively. Results from simple correlations indicated that PCV was negatively correlated with lnEPG (r = −0.255; r2 = 6.5%) and FAMACHA (r = −0.312; r2 = 9.7%), and positively correlated with BCS (r = 0.317; r2 = 10%). LnEPG was negatively correlated with BCS (r = −0.232; r2 = 5.4%) and PCV (r = −0.255; r2 = 6.5%), but positively correlated with FAMACHA (r = 0.178; r2 = 3.2%) and DS (r = 0.086; r2 = 0.7%). Results from the models indicated that PCV and lnEPG residuals were negatively correlated with FAMACHA, FCS and almost all categories of BCS and DS, although the correlations were very low. The main results from this study suggested that none of the subjective clinical parameters evaluated were highly correlated with PCV or lnEPG and therefore were not good predictors of lnEPG or PCV on the studied farms in Ontario and Quebec.
© 2014 Elsevier B.V. All rights reserved MenosABSTRACT.
A study was conducted in sheep on Canadian farms to describe the relationship between packed cell volume (PCV) or fecal egg counts (FEC) and subjective clinical parameters that
may indicate the severity of parasitic gastroenteritis. Twenty-one farms in Ontario (ON) and 8 farms in Quebec (QC) were purposively selected and visited during April?May (spring)
and August (summer) 2007. At each farm visit, blood and fecal samples were collected from 10 ewes and 10 female lambs; body condition score (BCS), dag score (DS), fecal consistency score (FCS) and FAMACHA score were recorded for all sampled sheep. Packed cell volume was determined for all blood samples, and FEC were performed for all fecal samples. Summary statistics and simple correlations were performed for the parameters recorded. Two mixed models with random effects at the farm level were developed; one using PCV as the response variable and another using the natural log of eggs per gram of feces (lnEPG). Finally, the residuals from both models were correlated to the covariates in the models. The mean PCV values during the spring were 29.7% and 36.7% for lambs, and 28.8% and 31.1% for ewes, in ON and QC, respectively. During the summer, the mean PCV was 32.0% and 32.8% for lambs, and 30.1% and 29.9% for ewes, in ON and QC, respectively. The arithmetic mean FEC per gram of feces (EPG) during the spring was 3 and 2 for lambs, and 1266 and 789 for ewes, in ON and QC, respectively, whereas during summer the arithm... Presentar Todo |
|
Palabras claves : |
NEMATODOS GASTROINTESTINALES. |
|
Thesagro : |
OVINOS. |
|
Asunto categoría : |
--
L73 Enfermedades de los animales |
|
Marc : |
LEADER 03399naa a2200265 a 4500
001 1053920
005 2019-10-15
008 2014 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $a10.1016/j.vetpar.2014.08.030$2DOI
100 1 $aMEDEROS, A.
245 $aEvaluation of the utility of subjective clinical parameters for estimating fecal egg counts and packed cell volume in Canadian sheep flocks.$h[electronic resource]
260 $c2014
500 $aArticle history: Received 12 February 2014 / Received in revised form 8 August 2014 / Accepted 30 August 2014.
520 $aABSTRACT. A study was conducted in sheep on Canadian farms to describe the relationship between packed cell volume (PCV) or fecal egg counts (FEC) and subjective clinical parameters that may indicate the severity of parasitic gastroenteritis. Twenty-one farms in Ontario (ON) and 8 farms in Quebec (QC) were purposively selected and visited during April?May (spring) and August (summer) 2007. At each farm visit, blood and fecal samples were collected from 10 ewes and 10 female lambs; body condition score (BCS), dag score (DS), fecal consistency score (FCS) and FAMACHA score were recorded for all sampled sheep. Packed cell volume was determined for all blood samples, and FEC were performed for all fecal samples. Summary statistics and simple correlations were performed for the parameters recorded. Two mixed models with random effects at the farm level were developed; one using PCV as the response variable and another using the natural log of eggs per gram of feces (lnEPG). Finally, the residuals from both models were correlated to the covariates in the models. The mean PCV values during the spring were 29.7% and 36.7% for lambs, and 28.8% and 31.1% for ewes, in ON and QC, respectively. During the summer, the mean PCV was 32.0% and 32.8% for lambs, and 30.1% and 29.9% for ewes, in ON and QC, respectively. The arithmetic mean FEC per gram of feces (EPG) during the spring was 3 and 2 for lambs, and 1266 and 789 for ewes, in ON and QC, respectively, whereas during summer the arithmetic mean EPG was 907 and 237 for lambs, and 458 and 246 for ewes, in ON and QC, respectively. Results from simple correlations indicated that PCV was negatively correlated with lnEPG (r = −0.255; r2 = 6.5%) and FAMACHA (r = −0.312; r2 = 9.7%), and positively correlated with BCS (r = 0.317; r2 = 10%). LnEPG was negatively correlated with BCS (r = −0.232; r2 = 5.4%) and PCV (r = −0.255; r2 = 6.5%), but positively correlated with FAMACHA (r = 0.178; r2 = 3.2%) and DS (r = 0.086; r2 = 0.7%). Results from the models indicated that PCV and lnEPG residuals were negatively correlated with FAMACHA, FCS and almost all categories of BCS and DS, although the correlations were very low. The main results from this study suggested that none of the subjective clinical parameters evaluated were highly correlated with PCV or lnEPG and therefore were not good predictors of lnEPG or PCV on the studied farms in Ontario and Quebec. © 2014 Elsevier B.V. All rights reserved
650 $aOVINOS
653 $aNEMATODOS GASTROINTESTINALES
700 1 $aKELTON, D.
700 1 $aPEREGRINE, A.S.
700 1 $aVANLEEUWEN, J.
700 1 $aFERNÁNDEZ, S.
700 1 $aLEBOEUF, A.
700 1 $aMENZIES, P.
700 1 $aMARTIN, R.
773 $tVeterinary Parasitology, 2014$gv.205, p. 568?574.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
